Dr. santosh medical awareness campaign's video: AFB ZN Stain Test For TB Acid Fast Bacilli Test in Hindi afb Stain Full form Use and Procedure

AFB / ZN stain test for TB | acid fast bacilli test in hindi | afb stain full form use and procedure ] Principle of Acid-Fast Stain When the smear is stained with carbol fuchsin, it solubilizes the lipoidal material present in the Mycobacterial cell wall but by the application of heat, carbol fuchsin further penetrates through lipoidal wall and enters into cytoplasm. Then after all cell appears red. Then the smear is decolorized with decolorizing agent (3% HCL in 95% alcohol) but the acid fast cells are resistant due to the presence of large amount of lipoidal material in their cell wall which prevents the penetration of decolorizing solution. The non-acid fast organism lack the lipoidal material in their cell wall due to which they are easily decolorized, leaving the cells colorless. Then the smear is stained with counterstain, methylene blue. Only decolorized cells absorb the counter stain and take its color and appears blue while acid-fast cells retain the red color. ★ Staining Procedure of Acid-Fast Stain •Prepare bacterial smear on clean and grease free slide, using sterile technique. •Allow smear to air dry and then heat fix. Alcohol-fixation: This is recommended when the smear has not been prepared from sodium hypochlorite (bleach) treated sputum and will not be stained immediately. M. tuberculosis is killed by bleach and during the staining process. Heat-fixation of untreated sputum will not kill M. tuberculosis •Cover the smear with carbol fuchsin stain. •Heat the stain until vapour just begins to rise . Do not overheat. Allow the heated stain to remain on the slide for 5 minutes. Heating the stain: Great care must be taken when heating the carbol fuchsin especially if staining is carried out over a tray or other container in which highly fiammable chemicals have collected from previous staining. Only a small fiame should be applied under the slides using an ignited swab previously dampened with a few drops of acid alcohol or 70% v/v ethanol or methanol.  •Wash off the stain with clean water. •Cover the smear with 3% v/v acid alcohol for 5 minutes or until the smear is sufficiently decolorized, i.e. pale pink. •Wash well with clean water. •Cover the smear with methylene blue stain for 1–2 minutes, using the longer time when the smear is thin. •Wash off the stain with clean water. •Wipe the back of the slide clean, and place it in a draining rack for the smear to air-dry •Examine the smear microscopically, using the 100 X oil immersion objective ★What do the results mean? If your results on an AFB smear were negative, you probably don't have active TB. But it could also mean there weren't enough bacteria in the sample for your health care provider to make a diagnosis. If your AFB smear was positive, it means you probably have TB or other infection, but an AFB culture is needed confirm the diagnosis. Culture results can take several weeks, so your provider may decide to treat your infection in the meantime. If your AFB culture was positive, it means you have active TB or another type of AFB infection. The culture can identify which type of infection you have. Once you have been diagnosed, your provider may order a "susceptibility test" on your sample. A susceptibility test is used to help determine which antibiotic will provide the most effective treatment. टेस्टइनहिंदी Topic explained in this video: 1• afb smear test, afb staining in hindi, 2• afb staining procedure, afb test cost, 3• afb test negative, afb test positive means, 4• afb test kya hai in hindi, zn staining test, 5• zn staining procedure, acid fast bacilli test, 6• acid fast bacilli color, 7• acid fast bacilli in sputum, 8• ziehl neelsen staining method, 9• sputum afb test for tb, afb culture test for tuberculosis, 10• acid fast bacilli test for tb,