Science Insights's video: PCR and RT-PCR Test Explained

What is PCR Test? Tamil What is Polymerase Chain Reaction? Tamil How PCR works Difference between PCR and RT-PCR What is PCR Testing for Viruses Our channels for +1 and +2 students- Biology Lectures and NEET Aspirants NEET Biology Tamil (Tamil Channel): https://www.youtube.com/channel/UCUHRy_nbdOawEGJCMillIFQ NEET Biology Expert (English Channel) : https://www.youtube.com/channel/UCkbHIv9PzhGyVxa7sGi9JpA This video explains in detail about PCR and RT-PCR tests in Tamil. PCR stands for Polymerase Chain Reaction. PCR is a major molecular biological technique used to amplify DNA. Dr. Kary B Mullis, an American Biochemist invented PCR. Applications of PCR: In the diagnostic field, Biomedical Research, Forensic Sciences, Parenting disputes, etc. Types of PCR: Real-time quantitative PCR, Reverse transcriptase RT-PCR, Nested PCR, Hot start PCR, Multiplex PCR etc. Agarose gel Electrophoresis technique is used to visualize the amplified DNA in PCR. Reverse Transcription Polymerase Chain Reaction (RT-PCR) is used to detect and amplify Viral RNAs. RT-PCR is an accurate method than a rapid kit test for viruses. Requirements for PCR test: Template DNA, Primers, Taq Polymerase enzyme, Nucleotide bases, Buffer, and Thermal Cycler. PCR cycles: A typical PCR takes place in an automated thermal cycler machine consisting of a series of 20–40 repeated cycles with consistent temperature changes. In each cycle, there are three steps called the initial denaturation (94°C–98°C), annealing (50°C–65°C), and extension (72°C) Sources Credit: https://www.youtube.com/watch?v=_Fd7qQRebXk https://pixabay.com/ Watch Our Other Medical Science Videos in Tamil Human Anatomy & Physiology : https://www.youtube.com/watch?v=bEgYK72UEMw&list=PLWV95ev_EVpcGLK5zRbfqA_jbEjeCgdQN Immunology Videos: https://www.youtube.com/watch?v=AzyAV2GU2TI&list=PLWV95ev_EVpcter1hcTCdwj6qbnKND7ig Blood and Blood Groups: https://www.youtube.com/watch?v=tcFflazZZ3c&list=PLWV95ev_EVpdb8Y1LIVh8Or8gteCcsJ43 Pharmacology Videos: https://www.youtube.com/watch?v=r8hdEyaAJDw&list=PLWV95ev_EVpcBwcrrapZCj-mU2tQNAyLv Microorganisms (Bacteria/ Virus/Fungi): https://www.youtube.com/watch?v=XEXkQH9k35g&list=PLWV95ev_EVpehDN31wOTBpimL3qCAi_4z Microbiology Videos: https://www.youtube.com/watch?v=k33ykaloUKc&list=PLWV95ev_EVpeK5Dh4CLnwBoSNZtQX6rQ8 Molecular Biology & Biotechnology : https://www.youtube.com/watch?v=tIT518Q6k6o&list=PLWV95ev_EVpdhLbIYY9WwY1bsVQw42u_c Health Awareness Videos: https://www.youtube.com/watch?v=KsLYjB5IIwM&list=PLWV95ev_EVpe0rfnFW5Sh2VIpqUkYz7P8 Disclaimer: All the information mentioned in this video is only for educational purposes and doesn't advocate any other consult or opinion. So consult your doctor for any procedures and clear knowledge. பயனுள்ள மருத்துவ மற்றும் அறிவியல் விஞ்ஞானத்தை எளிய தமிழில் மக்களுக்கு சேர்ப்பது எங்களின் நோக்கம். இந்த வீடியோவை உங்கள் நண்பர்களுடன் பகிரவும். MEDICAL SCIENCE VIDEOS - தமிழில் by Science Insights