Solution- Pharmacy's video: Classification of Chromatography Part 03 Gel chromatography Basics of Phytochemistry Part 15

Download the "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Tests and other GPAT Materials - https://play.google.com/store/apps/details?id=co.bolton.nqilv 1. Basics of Phytochemistry (12) = Introduction and History of Chromatography https://youtu.be/nEnX6pna2p4 2. Basics of Phytochemistry (13) = Classification of Chromatography (Part 01) https://youtu.be/SVASSry_igM 3. Classification and Application of Chromatography (Part 02) | Basics of Phytochemistry (Part 14) https://youtu.be/oPGf90j1sy8 Classification and Application of Chromatography Chromatography is the technique is used primarily for the separation of the components of a sample in which the components are distributed between two phases of which one is stationary (Solid/ liquid) while other is mobile phase (liquid/ gaseous). These are as Paper, Column, TLC, HPTLC, HPLC, GLC, GSC, Ion exchange chromatography, Gel chromatography etc. All chromatographic techniques are useful for Qualitative as well as Quantitative applications. It has been one of the most useful techniques of general application not only to the field of phytochemical analysis, but also to a host of other biochemical processing sectors requiring rapid and efficient resolution of complex mixtures of chemicals. Based on the mechanism of separation: The method of chromatography uses various types of mechanisms to separate analyte. On the basis of mechanism of separation, it is of following types: 1. Partition chromatography 2. Adsorption chromatography 3. Size exclusion chromatography 4. Affinity chromatography 5. Ion exchange chromatography Gel Chromatography/Size Exclusion Chromatography In exclusion chromatography, the separation of molecules is based on size and shape. Principle 1. Large molecules, cannot enter any pores in the beads, so they are excluded and come down rapidly means move more rapidly through the column, and emerge (elute) sooner. 2. Medium-sized molecules can enter the larger size pores in the matrix, so they reach the end of the column later. 3. Small molecules, can enter through all pores of the beads so that their speed is retarded and comedown slowly and they have the largest volume to pass through before emerging from the column, elute last. The degree of retardation of a molecule is proportional to the time it spends inside the gel pores, which is a function of the molecule’s size and the pore diameter. Get in touch with the solution by just clicking the following links- Facebook Group- https://www.facebook.com/groups/solutionpharamcy Mobile App - https://play.google.com/store/apps/details?id=co.bolton.nqilv New Channel (Pharmacy Dictionary) https://www.youtube.com/channel/UCt6OXVV_2oxf5DD0Mad6e9A E-Mail for official and other work - solutionpharmacy@gmail.com