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VascoElbrecht's video: Developing fusion primers for DNA metabarcoding - sample indexing tagging Illumina

@Developing fusion primers for DNA metabarcoding - sample indexing / tagging (Illumina)
In this tutorial we are looking a fusion primer design, to include amplification primers, inline tagging, and Illumina sequencing adapters/flow cell binds in a single primer set, to reduce tag switching and allow for target amplification and library preparation in the same single PCR step. 0:01 quick introduction into DNA metabarcoding 2:41 Illumina index system explained 7:00 Short fusion primer introduction 8:11 Skip the index read step when sequencing with fusion primers on MiSeq / HiSeq 9:16 Exploring fusion primers, sequence shifting and parallel sequencing (adds diversity) 14:57 Why is sequence diversity important, how to design inline tags 18:50 Visualisation (shifting and parallel sequencing) 22:20 Primer combinations 32:20 One and two-step PCR approaches 27:10 Summary and pro/cons of using fusion primers for metabarcoding Literature and further reading: * HCO/LCO Fusion primers discussed, parallel sequencing (http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130324) * Tag switching with fusion primers (Figure S4 http://onlinelibrary.wiley.com/doi/10.1111/2041-210X.12789/full) * Video on Illumina sequencing: https://www.youtube.com/watch?v=fCd6B5HRaZ8

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This video was published on 2017-11-07 06:35:39 GMT by @luckylion on Youtube. VascoElbrecht has total 9.2K subscribers on Youtube and has a total of 63 video.This video has received 64 Likes which are lower than the average likes that VascoElbrecht gets . @luckylion receives an average views of 34.9K per video on Youtube.This video has received 5 comments which are lower than the average comments that VascoElbrecht gets . Overall the views for this video was lower than the average for the profile.

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